edu) to be added as a trained user in iLab. 5 25 75 25 50 50 50 100 0 100 结合反应 Table 2. The SpectraMax® M3, M4, M5 and M5e readers offer a modular, upgradeable dual monochromator microplate reader platform with a wide range of high performance multi. Plates are used to validate optical performance of SpectraMax M3, M4, and M5/M5e readers. Microplate readers from Molecular Devices are designed to provide consistent performance for many years. The LMax plate reader has similar calculations and its RLUs are comparable to those of LMax II 384. 00 USD. TheSpectraMax®M3Multi-ModeMicroplateReader,SpectraMax®M4Multi-ModeMicroplate Reader,SpectraMax®M5Multi-ModeMicroplateReader,andSpectraMax®M5eMulti-Mode MicroplateReaderaredualmonochromator,multi-detectioninstrumentswithatriple-mode cuvetteportanda6-wellto384-wellplatereadcapability. Incubation. B: Selection of Excitation Wavelength. Choose from a three- (M3), four- (M4), or The SpectraMax ® M3, M4, M5, and M5 e Microplate Readers are a series of dual-monochromator, multidetection, multi-mode instruments with a triple-mode cuvette port and 6-well to 384-well microplate reading Products : SpectraMax i3x reader, SpectraMax iD5 reader, SpectraMax iD3 reader, General, FilterMax F5 reader, SpectraMax ABS reader, SpectraMax L reader, SpectraMax M5/M5e reader This page contains the table of contents of resources available for the SpectraMax Microplate Reader Suite of Products. The spectrophotometric. Here, we illustrate how the SpectraMax iD5 reader, in combination with SoftMax ® Pro Software, is used to quantitate a cellular protein sample with two different kits, Pierce BCA Protein Assay Kit and Pierce Rapid. This assay is based on the disappearance of hydrogen peroxide (H 2 O 2) at 240 nm in a medium containing 20 mM H 2 O 2, 0. Step 3: Use the Template Editor in the cell standards section of the protocol to create a template of the assay indicating the location of standards (cell numbers for each dilution. AquaMax® 4000. The dried paper microwell plate was then placed in the SpectraMax M-5 microplate reader, and the signal intensities were measured for each well. The SpectraMax M5e Multi-Mode Microplate Reader features a triple-mode…. free tracer decreases, resulting in an. The chamber contains springs that position the cuvette in the proper alignment for a read. Microplate Readers Molecular Devices Spectramax iD3 Microplate Reader. 2. 96-1536. Description 2 SpectraMax M2 & SpectraMax M2e Multi-mode Plate Readers Operator’s Manual. Recommended Optics Wavelength (nm) Wavelength. or multimode readers such as the SpectraMax M5, Analyst HT or Analyst GT. TR-FRET assay. 6: bioNova científica, s. AdThe SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance and can be equipped to read volumes as low as 2uL in one multimode reader package. For the Live/Dead assay, they were treated. 00 USD. Introduction. It should work on the Spectramax L reader and also on the Spectramax M3, M4, and M5 plate readers (new firmware only) with Softmax Pro ® software version 5. keeping with best practices, reader performance should. 95. Molecular Devices Spectramax M5 Microplate Reader. 00 USD. Choose from a three- (M3), four- (M4), oron the SpectraMax Multi-Mode Microplate Readers by issuing a single. The Molecular Devices SpectraMax M5 is a multi-mode plate reader that delivers single mode reader performance with five preferred detection modes for a wide range of applications. 1–0. on Molecular Devices microplate readers: SpectraMax® iD5, i3x, Paradigm®, and M5 Multi-Mode Microplate Readers. When a SOP Title: Use and Maintenance of a Molecular Devices M5 Plate Reader Document ID: HSL_EQ_005 Version 2. The Spectr. for the SpectraMax M3/M4 and FlexStation® 3 Multi-Mode Microplate Readers. Add to Cart. 2 Figure 3 shows the same assay plate read on the Analyst HT multi-mode reader. book Page 1 Wednesday, July 7, 2010 2:09 PMThe SpectraMax M2/M2e User Guide is a comprehensive manual that provides detailed instructions on how to operate, maintain, and troubleshoot the SpectraMax M2 and M2e microplate readers. 5 units/mL yielded a delta mP of 303, with a Z’ factor of 0. 1. The SpectraMax® Glo Steady-Luc™ Reporter Assay Kit provides a highly sensitive assay for the quan-titation of firefly luciferase expression in mammalian cells. SpectraMax® M4. However, when a specific probe is not available, or it causes off-target effects on the. Parallel negative control assays had primary, secondary, or both antibodies omitted. 3 Version 7. analysis, controls the SpectraMax Multi-Mode Microplate Readers. If you do not have an iLab account you must first make one: would like to show you a description here but the site won’t allow us. Ltd. Plate Shaking. The temperature of the microplate chamber can be regulated, if desired from 4°C above ambient to 45°C. Detection of pro-inflammatory factorsThe SpectraMax® i3 Multi-Mode Detection Platform comes with standard spectral absorbance, fluorescence, and luminescence detection. SpectraMax M Series Multi-Mode Microplate Readers 7 The SpectraDrop Micro-Volume Microplate offers the ability to use as little as 2µL samples with 24- or 64-well plates. Products : SpectraMax i3x reader, SpectraMax iD5 reader, SpectraMax iD3 reader, General, FilterMax F5 reader, SpectraMax ABS reader, SpectraMax L reader, SpectraMax M5/M5e reader This page contains the table of contents of resources available for the SpectraMax Microplate Reader Suite of Products. Our featured SpectraMax® L Microplate Reader Sensitive luminometer with programmable injector options for 96- and 384-well microplates. Microplate formats. The concentration of antibodies was determined by using a spectrophotometer from Puxi General Instrument Co. Representative data from the SpectraMax i3x Multi-Mode Microplate Reader for both MycoAlert and MycoAlert PLUS assays are shown in Figure 3. 0112-0126B 8 Chapter1:Introduction TheSpectraMax®340PC384,SpectraMax®190,andVersaMax™microplate. Put 200 µL sample into one or more wells of a microplate and 200 µL buffer or solvent into one or more other wells. When the plate was read on the SpectraMax M5 microplate reader, an enzyme concentration range of 0. A microplate reader asThe SpectraMax® Quant™ dsDNA Assay Kits are designed for fluorescence-based dsDNA quantitation across a broad range of concentrations. One vial of Thaw-and-Use TIGIT Effector Cells was thawed and resuspended in cell recovery. Molecular Devices. spectramax m5 multi. Plates are used to validate optical performance of SpectraMax M3, M4, and M5/M5e readers. SpectraTest Validation Plates form Molecular Devices such as ABS1 absorbance, FL1 fluorescence & LM1 luminescence validation plates are the valuable tools for verifying that absorbance, fluorescence, and luminescence-capable microplate readers are operating correctly for GLP and GMP laboratories. The SpectraMax® M2 and M2e readers provide two detection modes in one platform, and offer the the added functionality of bottom-read fluorescence for cell-based assays (M2e). The Gemini XPS Reader can be integrated with Molecular Devices’ StakMax® Microplate Stacker in a matter of minutes and begin reading microplates with seven mouse clicks. Figure 3. The bound bio-peptidomimetics were recognized by Eu 3+-SA (diluted using 1% BSA in TBS), and the fluorescence intensities were measured using a SpectraMax M5 microplate reader after adding 200 μL signal enhancement solution, which the parameters were set as the followed: excitation and emission wavelengths of 340 and 615 nm,. SpectraMax® M4. SoftMax® Pro Software for Windows 10 and 11 is designed to provide the simplicity, flexibility and power required for advanced data analysis. played in a grid format that corresponds to the wells in a microplate (all instruments) or individual cuvettes (using SpectraMax Plus, Plus384, M2, M2e, M5e or M5). Absorbance Detection Cartridges combine scanning and broad spectrum wavelength range to address a variety of nucleic acids, proteins, ELISAs and immunoassays found in the laboratory. This document is copyright protected and any reproduction of this document, in whole or any part, is strictly prohibited, except as Molecular Devices may authorize in. The SpectraMax M5 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes, 96-, and 384. 04 to 3. Please refer to the Obsolete Microplate Reader Models for more information. Page 1 of 8 . Protocol Name Version 6. Lift up the lid over the chamber to insert or remove a cuvette. Turn on the microplate reader. The Multiskan Sky microplate reader is an absorbance plate reader. The absorbance was measured at 570 nm using a SpectraMax M5 microplate reader (Molecular Devices, Sunnyvale, CA). Taurine ≥99 % was used as standard (Sigma-Aldrich ®,. The 1/absorptivity value for double stranded DNA at 260 nm is commonly assumed. After that, the cells were resuspended and diluted to a suitable density using DCFH-DA working solution, transferred to an opaque 96-well plate and incubated for 30 min. Tunable wavelength selection / monochromator. This application note discusses the principles on which PathCheck iswells in a microplate (all instruments) or individual cuvettes (using SpectraMax Plus, Plus384, M2, M2e, M5e or M5 readers). Ltd. In SOFTmax PRO, set up a plate section for an excitation scan with no cutoff filter and use the medium PMT setting. Molecular Devices Corporation have recently introduced the SpectraMax® M5e multi-detection microplate reader, an extension of the five-mode SpectraMax M5 system. The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. 6-384 (96- and 384-well with pipetting) 6-384. on the SpectraMax Multi-Mode Microplate Readers by issuing a single. Fill a clean plate with water. Read More. Learn how to set up protocols, customize reports, export results, and more with this PDF document. When the user applies a set temperature of 37°C, the top is heated to 37. 3 or higher) SpectraMax® M5 and M5e Multi-Mode Microplate Readers; SpectraMax® M4 Multi-Mode Microplate Reader;. For more detailed information and technical support of Life Technologies assays, please call 1-800-955- 6288 and enter extension 40266 or email drugdiscoverytech@lifetech. Molecular Devices SpectraMax M5 Multimode Microplate Reader. Molecular Devices Spectramax M5 Microplate Reader. Flurescence intensities were read using a Spectramax M5 microplate reader (Molecular Devices, Sunnyvale, CA, USA). Depending on which. The following document is intended to demonstrate setup of this instrument. CAT activity was measured using SpectraMax M5/M5 Microplate Reader (Molecular Devices, MDS Analytical Technologies). Incubation to 70 o C enables high temperature assays such as enzyme kinetics, protein stability/aggregation. Cuvette port functionality can also be controlled using SoftMax Pro Software. Microplate Readers Biotek Epoch Microplate. SoftMax Pro Software is an industry-leading acquisition and analysis software with over 160 ready-to-run protocols, powerful data analysis, and a range of data output options. SpectraMax® Microplate Readers and SoftMax® Pro Software. Note: In this user guide, SpectraMax ® refers to several SpectraMax . 305 reference level saturation. Products : SpectraMax 250 (Obsolete) SpectraMax® 250 User Manual Download Page. The cuvette must be in place when you read the plate. It is upgradeable to include a barcode reader, programmable injectors, and dual-channel configurations for a 2-fold increase for medium throughput. Examples: “Press carefully to engage,” “ Do not. 0 and 0. AquaMax® 2000. Figure 1. The purpose of this procedure is to set instructions in the proper use and handling of the Molecular Devices M5 Plate Reader. 7°C (gradient heating design). 2. Molecular Devices SpectraMax M2e Multi-Mode Microplate Reader w/ SoftMax Pro. ppr - M3, M4, M5, M5 SpectraMax readers (in SMP 4. These instruments often feature additional detection modes in addition to fluorescence intensity, such as UV-vis absorbance, fluorescence. 04 to 3. When a feature or capability applies to only certain readers, this exception is noted. Lift up the lid over the chamber to insert or remove a cuvette. SpectraMax M3, M4, M5, and M5 e Multi-Mode Microplate Readers 0112-0115 F 9 PathCheck ® Pathlength Meas urement Technology A SpectraMax Multi-Mode Microplate Reader with PathCheck Pathlength Measurement Technology allows normalization of variable well volumes to 1-cm cuvette readings. 05 3. Add to Cart. In these readers, RLUs are proportional to counts/sec which doesn't change with integration time assuming the signal is constant. Microplate Reader, MultimodeProducts : SpectraMax M2/M2e reader, SpectraMax Plus 384 reader, SpectraMax M4 reader, SpectraMax M5/M5e reader, SpectraMax M3 reader. Filterless readers provide consistent results with steady temperature regulation. The cuvette must be in place when you read the plate. Key applications: ELISA, nucleic acid, endotoxin testing. The fluorometric performance of the SpectraMax M2 e is similar, but slightly superior to that of the Gemini EM, a dedicated top and bottom read fluorescence microplate reader. Label-free detection. The SpectraMax® M3 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes and 6-, 12-, 24-, 48-, 96-, and 384-well microplates. ; Page 2 Molecular Devices equipment, software, reagents, and consumables. The SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. The CellTiter-Glo assay from Promega uses luciferase. Table of Contents. If you do not have an iLab account you must first make one: The Molecular Devices SpectraMax M3 Multi-Mode Microplate Reader is a dual-monochromator, multi-detection microplate reader with full spectral range detection for cuvettes and 6-, 12-, 24-, 48-, 96-, and 384-well microplates. Learn how to operate the VersaMax, SpectraMax 340PC384 and SpectraMax 190 Plus384 microplate readers, which offer fast and accurate absorbance measurements for a variety of assays. Preparation of Calibration Standards Calibration Standard (% Phosphorylated) µL Peptide Stock µL Phospho-peptide Stock 0 100 0 12. 5039228D December2018 SpectraMax® i3x Multi-ModeDetectionPlatform WiththeSpectraMax® MiniMax™300ImagingCytometer UserGuide SpectraMax® M5 Multi-Mode Microplate Reader (Molecular Devices P/N #M5) SpectraMax® Gemini™ EM Microplate Reader (Molecular Devices P/N #EM) Methods Instrument and protocol setup. SpectraMax 340PC384 Microplate Reader A versatile 96- and 384-well format absorbance reader let the monochromator tune to that exact wavelength. Figure 1. 7°C and the bottom to 36. The SpectraMax® M Series Multi-Mode Microplate Readers are modular and upgradeable with a wide range of high performance capabilities ideal for life science research and. M3 M4 M5 M5e LM1 v<nnn>. . The ultimate guide to microplate readers is an eBook that tells you everything you need to know about plate reader solutions from what a microplate reader is, to popular applications and technology, to GxP compliance and special tips and tricks to help maximize your. For readers without a cuvette port, these options are not shown in the software. User guide for SpectraMax M5 Microplate Reader Before beginning, you should contact Tatiana Laremore ([email protected] or greater. The SpectraMax M5 features a triple-mode cuvette port and a single-read command capability for experiments requiring multiple detection parameters. Wide range of applications. The instrument reads any standard cuvette, microcuvette, test tube or 96- and 384-well standard or UV transparent microplate over a full 190 to 1000. -Wide range of applications -Five preferred detection modes -Maximum signal with lowest background The dual monochromator optics allow the widest range of. Documentation Computer Integration Each Molecular Devices microplate reader is shipped with a license key for the SoftMax® Pro Data Acquisition and Analysis Software that you install on the computer that you use to operate the instrument. This reader's dual monochromators for. 1. SpectraMax ® M5 Multi-Mode Microplate Reader Transcreener ® ADP. The SpectraMax® M3 Multi-Mode Microplate Reader features dual monochromatic optics to allow the widest range of applications to be utilized for bioresearch procedures, without the need to change filters. SpectraMax L Microplate Reader is a sensitive luminometer with programmable injector options that measures flash and glow luminescence assays, including dual luciferase reporter gene, G protein-coupled receptor (GPCR) in 96- and 384-well plates. Standard features include a cuvette port, spectral scanning in 1 nm increments, and up to six wavelengths per read. The SpectraMax® L Microplate Reader measures flash and glow luminescence assays in both 96- and 384-well plates. By using PathCheck, SpectraMax readers can automatically normalize sample absorbance values to a 1-cm pathlength and calculate the concentrations. SpectraMax iD5; SpectraMax Mini; SpectraMax Mini Install Guide; SpectraMax Mini Reader Plug-In Download Page (required for SoftMax Pro 7. AlphaScreen and AlphaLisa detection using a 680 nm excitation laser diode and a 570/100 nm emission filter. Levels of immunoreactivity were normalized to. Fluorescence Microplate Reader Comparison Chart. Microplate Reader, Multimode Tecan Infinite M200 Pro Nanoquant M Nano+ Dual-Mode Microplate Reader 30050303. • Tel. A microplate reader that can detect two or more applications is considered a multi-mode plate reader. The spectrophotometric. This user guide provides detailed instructions, troubleshooting tips and technical specifications for these instruments. 1. The raw data from each individual experiment were normalized to vehicle-treated HK-2. Microplate Readers Biotek Synergy H1 Hybrid Microplate Reader. l. The SpectraMax® M Series Multi-Mode Microplate Readers are modular and upgradeable with a wide range of high performance capabilities ideal for life science research and drug discovery screening. SpectraMax M5e Multi-Mode Microplate Reader. All associated StakMax® stackers. The SpectraMax M5 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes, 96-, and 384-well microplates. com. This is equivalent to having 511 built-in filters,The SpectraMax M5 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes, 96-, and 384-well microplate. Temperature variation ± 0. Molecular Devices SpectraMax ® i3x MultiMode Microplate Reader, in combination with luminescent assays for cell viability and cytotoxicity, provides a sensitive and rapid way to measure the number of viable cells in culture and quantify the cytotoxic effects of experimental treatment. Choose from a three- (M3), four-. Call 516-939-0888 to check. Microplate Readers Biotek Synergy H1 Hybrid Microplate. SpectraMax® Plus 384 Absorbance Plate Reader, Molecular Devices. Analyst, M5, Flex 3, and SpectraMax L readers work differently. The dual monochromator optics allow. The plate reader can measure multiple readouts, including UV and visible absorbance. 0 out of 5 $ 2,499 $ 1,999. Analytical Technologies Group has deep experience maintaining and repairing Molecular Devices microplate readers, including the SpectraMax® M Series microplate readers (M2, M2e, M3, M5, M5, and M5e). After 24 h drug treatment, cells were lysed by RIPA buffer (Life Technologies Inc, US). 14 Average %CV 0. Figure 1. SpectraMax ® M5 Multi-Mode Microplate Reader Transcreener ® ADP. flexibility of monochromator-based systems. SpectraMax iD3 Multi-Mode Microplate Reader; Methods Cell treatment. The dual monochromator optics allow the. 00 USD. Learn how to set up protocols, customize reports, export results, and more with this PDF document. Microplate Readers include the M3, M4, M5, and M5e models. Step 2. The temperature of the microplate chamber can be regulated, if desired from 4°C above ambient to 45°C. It provides ready-to-run protocols, analysis algorithms, and 21 different curve fit options. Add to Cart. The SpectraMax® M3, M4, M5 and M5e Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science research and drug discovery screening. The SpectraMax® M3, M4, M5 and M5e Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science. 87. 49 2. Fluorescence microplate readers are multimode spectrophotometers with the capability to detect and measure fluorescence emitted by analytes in a multi-well sample format. Our absorbance plate readers feature our PathCheck Sensor. 5039228D December2018 SpectraMax® i3x Multi-ModeDetectionPlatform WiththeSpectraMax® MiniMax™300ImagingCytometer UserGuideSpectraMax® M5 Multi-Mode Microplate Reader (Molecular Devices P/N #M5) SpectraMax® Gemini™ EM Microplate Reader (Molecular Devices P/N #EM) Methods Instrument and protocol setup. SpectraMax M3, M4, M5, and M5e Microplate Reader User Guide Cuvette Chamber The cuvette chamber is located to the right of the control panel. , USA). Choose cables that meet the requirement described in this appendix. upgradeable with a wide range of high performance capabilities ideal for life science research and drug. FlexStation® 3 Multi-Mode Microplate Reader. The SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. The SpectraMax M5e Multi. Uncontrolled Copy for Training and Reference Purposes Only . A new microplate luminometry for the toxicity bioassay of chemicals on firefly luciferase, was developed using the multifunctional microplate reader (SpectraMax M5) to measure the luminous intensity of luciferase. System Diagrams and Dimensions. The optical density (OD) was measured at 450 nm with a SpectraMax M5 microplate reader (Molecular Devices). Manufacturer: Molecular Devices; Model: SpectraMax; The SpectraMax M5 Multi-Mode Microplate Reader delivers single mode reader performance in one multimode reader package. For fluorescence intensity, time resolved fluorescence, and fluorescence polarization assays, the SpectraMax M5 Microplate Reader optical design provides the highest level of flexibility. Molecular Devices. Label: provides information about the reader, such as line voltage rating, caution-ary information, serial number, etc. spectramax multi-mode microplate Readers The SpectraMax® M3, M4, M5 and M5 Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science research and drug discovery screening. Anne T. 2. 0. Bottom-reading will also give you higher RFU's (and the appearance of high sensitivity) because the polystyrene will fluoresce a bit. The SpectraMax® Plus 384 Microplate Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. SpectraMax M3, M4, M5 and M5e Multi-Mode Microplate Readers User Guide Fluorescence Microplate Reader Comparison Chart. The SpectraMax M2 and M2e are versatile and reliable. . The SpectraMax M5e Multi-Mode Microplate Reader features a triple-mode cuvette port, temperature control, microplate shaking, and the industry-leading SoftMax Pro. 1. Published Dec 16, 2020 | Updated Dec 16, 2020. For more detailed information and technical support of Life Technologies assays, please call 1-800-955- 6288 and enter extension 40266 or email [email protected]% Triton X‐100, 10 mM potassium phosphate buffer pH 7. The K d was calculated with GraphPad Prism software (GraphPad Software Inc. Category 2 and 3 microplate readers use gradient heating as well. The guide also covers the software features, the specifications, and the accessories of the systems. 6 for further experiment, so I'm confused which i would choose for OD value determination. 1 is GxP edition only; Protocols for the ABS1 Validation Plate. Conclusions We report a peptide-induced super-assembly of enzyme-containing MOFs for programmed enzyme. Molecular Devices LMax II Luminometer Luminescence Microplate Reader w WARRANTY $2,450. Between steps, reactions were rinsed 3 times with TBS + 0. SpectraMax® iD5. degree of automation, the Automation Vendor Partners Program has streamlined the integration of our microplate reader systems with all leading partner. The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. SpectraMax iD5 Multi-Mode Microplate Reader, a hybrid monochromator- and filter-based, five-mode plate reader with automatic filter identification and western blot capability. This application note describes experimental protocols that enable the detection of relatively low numbers of cells expressing Green Fluorescent Protein. The SPECTRAmax ® GEMINI-XS is a dual-monochromator microplate fluorometer system incorporating highly sensitive optics 4 that permit experimental determinations at low-level detection limits. The SpectraMax® Plus 384 Microplate Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. Figure 4: Transcreener ADP. View the Molecular Devices website. For more sample throughput, the system can Products : SpectraMax M2/M2e reader, SpectraMax M5/M5e reader, Gemini XPS reader, SpectraMax Plus 384 reader, SpectraMax 190 reader, VersaMax reader, FlexStation 3 reader I need to reinstall the USB driver for my plate reader. This monochromator-based instrument allows users to select optimal wavelengths for any chosen fluorophore without the need to purchase additional filter sets. We specialize in the full suite of AKTA™ FPLC systems, Molecular Devices SpectraMax™ microplate readers, BioTek microplate washers, YMC (formerly LEWA) Process Technologies systems, ChromaCon Contichrom® instruments, and. microplate reader. Cuvette port functionality can also be controlled using SoftMax Pro Software. 40266 Email: drugdiscoverytech@lifetech. 0 . Selection of Water Constant in PathCheck settings. As. edu) to be added as a trained user in iLab. Spectramax M5 Microplate Reader, supplied by Danaher Inc, used in various techniques. For biological applications involving small samples, such as purificationThe guidelines for cuvette use in SpectraMax instruments are the same as those that apply to any high-quality spectrophotometer. SpectraMax M5e Multi-Mode Microplate Reader. AlphaScreen assays are compatible with our SpectraMax i3x, SpectraMax i3, and SpectraMax Paradigm Multi-Mode Microplate readers with the Alphascreen Detection Cartridge options. The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance and can be equipped to read volumes as low as 2uL in one multimode reader package. Flexible for 6-well to 384-well microplates and cuvettes. Setup for GeneBLAzer® Assay on SpectraMax® M5/M5e Microplate Reader with SoftMax® Pro 6 Software The Molecular Devices SpectraMax® M5/M5e Microplate Reader was tested for compatibility with Life Technologies GeneBLAzer® assays. iD3, iD5, i3, i3x, M3, M4, M5, M5e, L, and FlexStation 3 readers. All assays were performed in at least three individual experiments, each comprising no. Wide range of applications The VersaMax reader covers. 1. Cell viability was assayed by addition of BacTiter-Glo ™ bioluminescence reagent according to the manufacturer’s protocol (Promega) and luminescence measured using a Spectramax M5 microplate reader (Molecular Devices). Are you using the correct Microplate Adapters? For the SpectraMax M3/M4/M5 readers - use the Purple Assay Plate adapter for Top Read only. (Beijing, China) and a BCA protein assay kit from Beijing Solarbio Technology Co. or multimode readers such as the SpectraMax M5, Analyst HT or Analyst GT. The instrument reads any standard cuvette, microcuvette, test tube or 96- and 384-well standard or UV transparent microplate over a full 190 to 1000. We use Spectramax M5 microplate reader in our lab. Five. The SoftMax® Touch. 1 Version 7. Add to Cart. Because many reagents can be fluorescently labeled, spectrofluorometers are used to detect many biological and chemical processes. Microbiota DNA was. The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance and can be equipped to read volumes as low as 2uL in one multimode reader package. 59 6. Trilevel commercial control reagents (StanbioThe SpectraMax® M3, M4, M5 and M5e Multi-Mode Microplate Readers are a modular, upgradeable dual- monochromator microplate reader platform offering a wide range of. 0. "With the launch of the Molecular Devices® SpectraMax® M3 and M4 Multi-Mode Microplate Readers, we now offer a full range of multi-mode detection capabilities within our SpectraMax® product line," said Andy Boorn, President of MDS Analytical Technologies. However, microplate optimization and read height adjustment should be performed any time a new plate type—or lot of plates—or reagent volume is. 2 only) SpectraMax M-series Microplate readers. The drawing shows a standard microplate of 15 mm height covered with a lid. The SpectraMax® Paradigm® Multi-Mode Microplate Reader measures absorbance, fluorescence, time-resolved fluorescence (including HTRF), fluorescence polarization, AlphaScreen®, AlphaLISA®, and luminescence assays for up to 1536-well plates. 1. 2. 5°C at 37°C. 8 µg pNF-κB-After 3 h of incubation at 37 °C, the medium was gently removed, and a 150-μL aliquot of DMSO was added to each well. Both readers feature an 8-channel read head for fast reads and. Every step is optimized for data acquired from a Molecular Devices microplate reader or data imported from. Tailored to your different needs, these assay kits are configured and optimized for Molecular Devices SpectraMax® microplate readers, with preconfigured protocols provided in SoftMax® Pro Software for. 315 can't find zero order. 0 out of 5 $ 16,499. The SpectraMax M2 is a dual-monochromator, multi-detection microplate reader with a dual-mode cuvette port and 96 or 384 microplate reading capability. This technical note demonstrates validation of the SpectraMax® i3x Multi-Mode Microplate Reader for NanoBRET™ assays. both cuvette-based and microplate reader applications on the same instrument. Read one sample or up to 384 inThe SpectraMax® M2 and M2e readers provide two detection modes in one platform, and offer the the added functionality of bottom-read fluorescence for cell-based assays (M2e). These robust readers have been placed in labs from Antarctica to the. Figure 1. Catalog Number: (89212-396) Supplier: Molecular Devices. We use Spectramax M5 microplate reader in our lab. or multimode readers such as the SpectraMax M5, Analyst HT or Analyst GT. 6 0. Description: This UV-visible absorbance microplate reader provides ultrafast, full spectral range detection for cuvettes, 96-well, and 384-well microplates, with t. Small, unbound molecules rotate rapidly in. Learn how you can utilize microplate readers to drive landmark discoveries. Launch SoftMax® Pro Software and open the PicoGreen fluorescence protocol from the Protocols dropdown menu. VersaMax Microplate Reader An affordable, tunable microplate reader for 96-well format Affordable The robot-compatible VersaMax reader is cost-effective—users never have to buy filters. Molecular Devices SpectraMax™ Plus 384 Microplate Reader (s) with One-Year Warranty $9,000. SpectraMax M5eは、宇宙に打ち上げられた世界初のマイクロプレートリーダーです！ NanoRacksとモレキュラーデバイスとのパートナーシップ、そしてSpaceX Commercial Resupply Services MissonでSpectraMax M5eを打ち上げた取り組みの詳細については、こちらをクリックしてください。Which SpectraMax Multi-Mode Microplate Reader do you need? Detection Modes Absorbance Fluorescence Intensity Luminescence Time-Resolved Fluorescence Fluorescence Polarization HTRF Upgrade Options Plate Formats 6-, 12-, 24-, 48-, 96-, 384-Well Microplates Certification and Validation IMAP Validation (TR-FRET only) (TR-FRET. While they play an important role in generating data from a wide range of. #SpectraMax L Config) Methods TIGIT/CD155 Blockade Bioassay. The SpectraMax ® i3x Multi-Mode microplate reader measures spectral-based Absorbance, Fluorescence, and Luminescence with the added functionality of. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A 7 1. Absorbance Verification Plate (Molecular Devices, Cat # 0200-6117) 6. metrically at 540 nm using a microplate reader (SpectraMax 384 Plus, Molecular Devices Corp. Standard SDS-PAGE was performed with a 10% (w/v) separating gel and a 4% stacking gel. We would like to show you a description here but the site won’t allow us. Molecular Devices LMax II Luminometer Luminescence Microplate Reader w WARRANTY $2,450. Offer Ends In: 44 Days 10: 22: 56. 3. Microplate Readers are modular and . The acceptance. The dual monochromator optics allow the widest range of applications to be utilized for bior. : 2 Oct 12 Page 2 of 10 Setup Guide on Molecular Devices SpectraMax® M5/M5e Microplate Reader Have a question? Contact our Technical Support Team NA: 800-955-6288 or INTL: 760-603-7200 ext. 67. analysis, controls the SpectraMax Multi-Mode Microplate Readers. e. Five preferred modes for diverse bioresearch applications. It provides ready-to-run protocols, analysis algorithms, and 21 different curve fit options. The CDWIA was performed on each. These lab workhorses are used in drug discovery, research, bioassay validation, and. Microplate Readers Fisher Scientific AccuSkan FC Microplate Reader – 2020. HTRF-compatible SpectraMax Multi-Mode Readers. e. 00. Figure 1. Maximum signal with lowest background. In SOFTmax PRO, set up a plate section for an excitation scan with no cutoff filter and use the medium PMT setting. 313 reference gain check fail. ) by nonlinear regression using. 1. Description: The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader. book Page 7 Wednesday, July 7, 2010 2:09 PM. These settings are also valid for the SpectraMax M3/M4 and FlexStation® 3 Multi-Modethe introduction of the SPECTRAmax PLUS microplate spectrophotometer and its PathCheck feature, the ability to determine the pathlength in each well of a microplate and automatically normalize the absorbance value to a 1 cm pathlength. Page 27 SpectraMax M2 and M2e Microplate Reader User Guide Place a standard 1 cm cuvette that contains the aqueous/solvent mixture you use for the plate samples into the cuvette port. The Molecular Devices SpectraMax M3 Multi-Mode Microplate Reader is a dual-monochromator, multi-detection microplate reader with full spectral range detection for cuvettes and 6-, 12-, 24-, 48-, 96-, and 384-well. Molecular Devices FlexStation™ 3 Multi-Mode Microplate Reader (Recent Model) with One Year Warranty (Svc Contract Available) Molecular Devices SpectraMax™ M5e Microplate Reader with 90 Day Depot Warranty (Svc Contract Available). Access this by going to Help >. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the. When the user applies a set temperature of 37°C, the top is heated to 37. 304 signal level saturation. SpectraMax Spectrophotometers for UV-Vis Absorbance Detection. With optimized reagents, option to operate the readers. Five preferred detection modes. 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